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Sponsor: University Hospital, Montpellier
Conditions: Rheumatoid Arthritis, Swelling Joint, Joint Fluid, Characterization of Regulatory T Lymphocytes
Interventions: Exploratory synovial fluid analysis
Countries: France
In rheumatoid arthritis (RA) the clinical response to anti-TNFα is related to an increase in the number or in the function of Treg lymphocytes in the peripheral blood of patients. This observation suggests the central role of Tregs in homeostasis of the immune response during RA. In the literature the Tregs frequency and phenotype in the peripheral blood are well documented, however the analyses done on the Tregs in inflamed environment are still fragmentary or disparate. In this project Tregs phenotype as well as expression of several transcripts will be analysed in order to better characterize the Treg cell subsets within the synovial fluid. Moreover, the local inflammatory cytokines (TNF, IL-6 and IL-1) may affect both the phenotype and the suppressive function of these Tregs and a comparison between peripheral and tissue Tregs will allow us to better understand the cause of functional loss. Outcomes: Primary outcome: Identification and characterization of the Tregs subpopulation present in the synovial fluid for RA patients suffering an episode of acute arthritis. Secondary outcomes: compare the phenotypic and expression profile of the Tregs present in the synovial fluid with the Tregs present in the peripheral blood of RA patients suffering from an episode of acute arthritis.
Sex: ALL
Age: 18 Years to 65 Years
Healthy volunteers: No
Study type: INTERVENTIONAL
Inclusion Criteria: * 18 to 65 years old * patients with rheumatoid arthritis according to 2010 ACR/EULAR criteria * Patients with swelling joint suffering from an episode of acute articular flare link to RA and that requires an aspiration of joint fluid (RA with less than 3 months of evolution confirmed by clinical examination and/or joint ultrasound) * Patients who agreed to participate to this study and signs the informed consent form Exclusion Criteria: * Septic arthritis confirmed by microbiological analysis of synovial fluid * Osteoarthritis of the reference joint confirmed by X-ray * Contraindication for joint aspiration: Platelets \< 50 000/mm3, Prothrombin time \<70%, Partial Thromboplastin Time Blood Test-PTT \> 1,5 times compare to the control sample, anticoagulant therapy * Biotherapy (including (anti-TNF-α), corticosteroid (orally, intravenously or intra-articulary) or others immunosuppressive drug (current treatment or treatment within 3 months before joint aspiration). * Pregnancy, mother in the post natal period or breastfeeding.
- Montpellier, France